METHOD OF DOUBLE CROSSOVER HOMOLOGOUS RECOMBINATION IN CLOSTRIDIA
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United States of America Patent
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Issued Date -
Apr 26, 2012
app pub date -
Jan 21, 2010
filing date -
Jan 22, 2009
priority date (Note) -
Abandoned
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Abstract
The invention relates to a method of double crossover homologous recombination in a host Clostridia cell comprising: a first homologous recombination event between a donor DNA molecule and DNA of the host cell to form a product of the first recombination event in the host cell, wherein the donor DNA molecule comprises a codA gene and at least two homology arms; and a second recombination event within the product of the first homologous recombination event, thereby to form a product of the second homologous recombination event in the host cell which is selectable by the loss of the codA gene; and a related vector and altered host cell.
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