Method for single oxygen atom incorporation into peptides
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United States of America Patent
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Mar 11, 2014
Grant Date -
Mar 29, 2012
app pub date -
Dec 5, 2011
filing date -
Nov 15, 2004
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Abstract
Optimized enzymatic conditions incorporate a single oxygen atom into digested peptides using a peptidase. The incorporation of a single oxygen atom is especially useful for proteolytic 18O labeling in comparative proteomics. The optimized proteolytic 18O labeling minimizes the generation of a mixture of isotopic isoforms of the peptides resulting from incorporation of either one or two 18O atoms. The outcome is accurate quantification of isotopically labeled peptides.
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