Method for Preparation and Purification of Recombinant Proteins
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United States of America Patent
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Issued Date -
Mar 1, 2012
app pub date -
Apr 2, 2004
filing date -
Mar 16, 2004
priority date (Note) -
Abandoned
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Abstract
The present invention relates to a method for the production, isolation, and purification of a recombinant protein, more particularly, to a method for isolating and purifying a foreign protein stably using Anti-Freeze Protein (AFP), thereby producing the protein. The present invention provides a method for the production, isolation and purification of a foreign target protein using its recombinant protein containing AFP, and a construct, an expression vector, a transformant and a recombinant protein. The recombinant protein produced by the present invention shows the biological property and function identical to a naturally occurring protein. Particularly, the present invention is advantageous for the expression and purification of a useful protein.
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