METHOD FOR GENERATING THROMBIN
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United States of America Patent
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Issued Date -
Feb 2, 2012
app pub date -
Jul 30, 2010
filing date -
Jul 30, 2010
priority date (Note) -
Abandoned
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Abstract
Methods of generating thrombin and methods of applying a clotting tissue sealant to a site on a subject are provided. A blood component comprising platelets can be obtained from the subject. A hypotonic composition is contacted with a solid matrix to form a thrombin-containing liquid, where the hypotonic composition includes water, calcium, a blood component comprising platelets, and optionally a chelator. Calcium is present in the hypotonic composition in an amount greater than the amount of calcium that can be complexed by the chelator. Thrombin-containing liquid is then separated from the hypotonic composition and can be applied to the site on the subject to form a clot, for example, by combination with fibrinogen.
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