Multiplex Nucleic Acid Detection

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United States of America Patent

APP PUB NO 20120021930A1
SERIAL NO

12085407

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Abstract

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The present invention relates to a novel multiplex detection of nucleic acid targets in a sample. This is accomplished by using novel padlock probes which contain a unique cleavage site for linearization and a first member of a binding pair for isolation of the padlock probe from the sample. Three different designs of the padlock probes are described and examples are shown for ligation, amplification and qualitative and quantitative detection. The combination of these features gives a fast, accurate and specific multiplex detection assay

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Patent Owner(s)

Patent OwnerAddress
STICHTING DIENST LANDBOUWKUNDIG ONDERZOEKDROEVENDAALSESTEEG 4 WAGENINGEN NL-6708 PB

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Bonants, Petrus Johannes Maria Rhenen, NL 1 120
Schoen, Cornelis Dirk Schagerbrug, NL 1 120
Szemes, Marianna Bristol, NL 1 120

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