ISOTHERMAL STRAND DISPLACEMENT AMPLIFICATION
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United States of America Patent
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Issued Date -
Jan 26, 2012
app pub date -
Jan 15, 2010
filing date -
Jan 21, 2009
priority date (Note) -
Abandoned
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Abstract
A method for isothermal DNA amplification comprising: providing to the DNA to be amplified an amplification mix comprising a first primer at least partially complementary to a region of DNA and containing Xanthosine, a second primer at least partially complementary to a region of DNA and containing Xanthosine, a DNA polymerase, an enzyme capable of strand displacement, an enzyme that recognises Xanthosine in double-stranded DNA and causes a nick or excises a base in one DNA strand at or near Xanthosine; and amplifying the DNA substantially without thermal cycling.
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