MATERIALS AND METHODS FOR ISOTHERMAL NUCLEIC ACID AMPLIFICATION
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United States of America Patent
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Issued Date -
Jan 26, 2012
app pub date -
Jan 7, 2011
filing date -
Jan 8, 2010
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Abandoned
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Abstract
A method for isothermal amplification of a target nucleic acid sequence is disclosed. The target nucleic acid is amplified by an enzyme with helicase activity and an enzyme with reverse transcriptase activity and DNA-dependant DNA polymerase activity. Also disclosed is a kit for isothermal amplification of a target nucleic acid sequence, including HPV nucleic acids. The kit comprises a first enzyme with helicase activity and a second enzyme having both reverse transcriptase activity and DNA-dependant DNA polymerase activity.
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