GROWTH SELECTION METHOD
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United States of America Patent
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Issued Date -
Jan 12, 2012
app pub date -
Jun 21, 2011
filing date -
Jun 21, 2001
priority date (Note) -
Abandoned
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Abstract
The invention provides growth selection methods for screening an environmental or a mixed population library of nucleic acids comprising uncharacterized nucleic acids for the presence of a nucleic acid encoding an enzyme of interest. In one aspect, methods for screening and identification of enzymes, e.g., transaminases, nitrilases, aldolases, epoxide hydrolases are provided. Methods for the production and screening of gene libraries generated from nucleic acids isolated from more than one organism for enzymes of interest, e.g., transaminases, nitrilases, aldolases, epoxide hydrolases, are also provided.
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