Methods for Recombinant Manufacturing of Anti-RSV Antibodies
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United States of America Patent
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Issued Date -
Jan 12, 2012
app pub date -
Sep 23, 2011
filing date -
Sep 7, 2007
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Abandoned
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Abstract
The invention relates to a method for manufacturing recombinant anti-RSV antibodies and antibody compositions. The method comprises obtaining a collection of cells transfected with a collection of variant nucleic acid sequences, wherein each cell in the collection is transfected with and capable of expressing one distinct anti-RSV antibody. The cells are cultured under suitable conditions for expression of the anti-RSV antibody/antibodies. The nucleic acid sequence is introduced into the cells by transfection with expression vectors, which avoid site-specific integration. The present method is suitable for manufacturing recombinant mono- and polyclonal anti-RSV antibodies for therapeutic uses.
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