METHOD FOR DETECTION OF AN ENZYME-SUBSTRATE REACTION
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United States of America Patent
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Issued Date -
Jan 12, 2012
app pub date -
Mar 18, 2010
filing date -
Mar 20, 2009
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Abandoned
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Abstract
The present invention relates to a method for detection of an enzyme-substrate reaction by capturing the product of the reaction on a solid support. More closely, the invention relates to a solid phase system for enzyme characterisation and screening of enzyme-targeted drugs. The method comprises the following steps a) rapidly mixing an enzyme solution with a substrate solution to start a reaction between enzyme and substrate wherein said reaction may result in product formation; and b) monitoring any binding of the product to a capturing agent immobilised on a solid surface, wherein said capturing agent is directed against the product of the enzymatic reaction. In a preferred embodiment the method comprises a further step c) measuring the initial rate of the product binding to the capturing agent and thereby characterize the enzymatic reaction. The method may also be used for drug screening.
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