SYSTEM AND METHOD FOR TAILORING NUCLEOTIDE CONCENTRATION TO ENZYMATIC EFFICIENCIES IN DNA SEQUENCING TECHNOLOGIES
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United States of America Patent
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Issued Date -
Nov 24, 2011
app pub date -
May 18, 2011
filing date -
May 21, 2010
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Abandoned
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Abstract
An embodiment of a method for optimizing sequencing performance is described that comprises the steps of calculating a nucleotide species specific degradation rate of an apyrase enzyme for a plurality of nucleotide species; determining a concentration for each of the nucleotide species using the nucleotide species specific degradation rate; iteratively providing the concentration of each of the nucleotide species in a reaction environment comprising a polymerase enzyme and a species of template nucleic acid molecule, wherein one or more molecules of the nucleotide species are incorporated into a nascent molecule in a sequencing reaction and the apyrase enzyme is introduced to the reaction environment to degrade unincorporated nucleotide species molecules; and detecting a signal in response to the incorporation of the nucleotide species.
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