METHOD AND APPARATUS FOR PERFORMING MULTIPOINT FCS
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United States of America Patent
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Issued Date -
Sep 22, 2011
app pub date -
Mar 15, 2011
filing date -
Mar 16, 2010
priority date (Note) -
Abandoned
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Abstract
A method of performing fluorescence correlation spectroscopy with a fluorescence microscope includes selecting an illumination area of a sample, generating an illumination light beam and splitting the illumination light beam into at least three partial beams. The partial light beams are focused onto the selected illumination area using a microscope optical system of the fluorescence microscope so as to excite fluorescent dye particles in the illumination area to fluoresce. Fluorescent light emitted by the dye particles is detected and at least one diffusion coefficient representative of a diffusibility of the fluorescent dye particles is determined based on the detected fluorescent light.
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