Nucleic acid extraction from complex matrices

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United States of America Patent

PATENT NO 8574890
APP PUB NO 20110201085A1
SERIAL NO

13028714

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Abstract

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The present disclosure describes an adsorbent and exemplary protocols for extracting nucleic acids, such as DNA and RNA, from complex matrices, such as stool samples and water samples. The adsorbent is activated charcoal coated with a material such as polyvinylpyrrolidone, dextran, or coconut flours. The adsorbent may be used in microcentrifuge spin columns, where it may be present as a slurry in a storage solution. The sample may be prepared by vortexing in a buffer solution, centrifuging, adding a protease to the supernatant, and passing the supernatant through a microcentrifuge spin column containing coated activated charcoal. The key components, including buffer, protease, and spin columns, may be packaged in a kit.

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Patent Owner(s)

Patent OwnerAddress
MICROBIOLOGICS INC200 COOPER AVENUE NORTH ST CLOUD MN 56303

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Icenhour, Crystal R Charlottesville, US 5 54
Loyal, Brian V Charlottesville, US 4 18
Nguyen, Linh N K Charlottesville, US 1 14

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