METHOD FOR MEASURING SURVIVIN mRNA
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United States of America Patent
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Issued Date -
Aug 4, 2011
app pub date -
Oct 6, 2009
filing date -
Oct 6, 2008
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Abstract
Disclosed is a method of amplifying and detecting mRNA of survivin gene in an RNA amplification process comprising: a step for forming a double-stranded DNA containing a promoter sequence by use of a combination of oligonucleotides consisting of a first primer having a sequence homologous with a portion of survivin mRNA and a second primer having a complementary sequence, wherein the promoter sequence is added to the 5′-end of either the first primer or the second primer with a reverse transcriptase, forming an RNA transcription product by use of an RNA polymerase with using the double-stranded DNA as template, and forming the double-stranded DNA by use of a reverse transcriptase by continuing to use the RNA transcription product as a template of DNA synthesis, measuring an amount of amplified RNA produced over time with an oligonucleotide probe designed so that signal properties change with the formation of a complementary double strand with the amplified RNA.
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