MODIFIED MULTIWELL PLATE FOR BIOCHEMICAL ANALYSES AND CELL CULTURE EXPERIMENTS.
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United States of America Patent
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Issued Date -
Jul 28, 2011
app pub date -
Dec 16, 2008
filing date -
Dec 19, 2007
priority date (Note) -
Abandoned
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Abstract
The invention relates to a modified multi-well plate for biochemical analyses and cell culture experiments, which can be obtained through a method for functionalization. The method comprises the following process steps: a) Treatment of the multi-well plate by means of an ammonia low-pressure plasma, so that reactive amino groups are formed on the plate surface; b) Application of an aqueous or alcoholic solution of a maleic anhydride copolymer; c) Drying the solution of the maleic anhydride copolymer on the surface; d) Heat treatment for the covalent bonding of the maleic anhydride copolymer to the multi-well plate; e) Rinsing with aqueous solution to eliminate unbound and water-soluble maleic anhydride copolymer f) Heat treatment to reestablish the reactivity of the anhydride groups on the maleic anhydride copolymer.
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