Analysing Methylation Specific PCR by Amplicon Melting
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United States of America Patent
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Issued Date -
Jul 14, 2011
app pub date -
Feb 27, 2009
filing date -
Feb 27, 2008
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Abandoned
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Abstract
The present invention provides a method of evaluating DNA methylation in a sample. The method comprises (i) reacting the DNA with an agent that differentially modifies methylated cytosine and non-methylated cytosine to produce modified DNA, (ii) amplifying the modified DNA by methylation specific PCR to produce amplified DNA, and (iii) subjecting the amplified DNA to melting analysis. In the method the methylation specific primers are selected such that the sequence between the primers includes a region of known sequence variation and/or at least one cytosine nucleotide.
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