Cloning Transcription Factor Gene OsWOX20 That Regulates The Growth and Development of Monocotyledon's Root and Uses Thereof
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United States of America Patent
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Issued Date -
Jun 30, 2011
app pub date -
Jun 26, 2008
filing date -
Jul 9, 2007
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Abandoned
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Abstract
he present disclosure pertains to the field of plant genetic engineering. Specifically, the present disclosure relates to isolation and cloning, function verification, and use of a transcription factor gene OsWOX20 that regulates growth and development of roots of monocotyledons. According to the present disclosure, a transcription factor gene Os WOX20 DNA is isolated which regulates growth and development of roots of rice, and has (a) the DNA sequence of positions 1-786 in SEQ ID NO: 1 in the Sequence Listing, or (b) a DNA sequence that encodes the same protein as that encoded by the DNA sequence of (a). The promoter according to the present disclosure has the DNA sequence of positions 1-2078 in SEQ ID NO: 3 in the Sequence Listing. The cloned gene sequence is used to transform a rice variety and transgenic rice plants showing markedly improved growth and development of roots are obtained. The promoter drives the specific expression of a reporter gene in the roots of rice. The present disclosure shows that the cloned target gene and its promoter have a great prospect in their use in breeding transgenic plants having improved growth and development of roots.
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