SEPARATING AGENT FOR PROTEIN PURIFICATION AND PROTEIN PURIFICATION METHOD
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United States of America Patent
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Issued Date -
May 5, 2011
app pub date -
Jun 22, 2009
filing date -
Jun 23, 2008
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Abstract
A novel separating agent for protein purification which not only can adsorb proteins in a sufficient amount for protein purification from a low concentration buffer but also can desorb the adsorbed protein easily just by altering the pH of the buffer and a simple and economical method for its production and a method for protein purification using it.One or two ligands selected from the group consisting of a ligand represented by the following formula (1):(wherein m is an integer of from 2 to 6) and a ligand represented by the following formula (2):alkyl group, and n is an integer of from 1 to 6) are immobilized on a support via a urethane bond without intervention of a spacer arm.
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