METHOD OF DISCOVERING AND ANALYZING SECRETED BIOMARKERS OF DISEASE FROM SOLID TISSUE
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United States of America Patent
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Issued Date -
May 5, 2011
app pub date -
Jun 23, 2008
filing date -
Jun 22, 2007
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Abstract
The current invention provides a method for discovering protein biomarkers of disease for use in diagnostic assays of bodily fluids by determining differential expression patterns of proteins secreted or released directly by normal and diseased epithelial cells into glandular lumens. Determining those secreted or released proteins directly from the glandular lumen of diseased and normal solid tissue would lead to a catalogue of proteins that have a high degree of probability to be present in various bodily fluids in persons with specific diseases. This would prove useful as a means to diagnose specific conditions and diseases by simply assaying easily acquired bodily fluids. Past efforts to discover such diagnostic/screening markers in bodily fluids have proven difficult at best due to overriding complexity of the proteins within bodily fluids. This invention is a method of discovering those biomarkers in a more focused and less complex protein subpopulation, namely the secreted or released proteins present in glandular lumens.
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