QUANTITATIVE NUCLEASE PROTECTION SEQUENCING (qNPS)
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United States of America Patent
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Issued Date -
May 5, 2011
app pub date -
Nov 3, 2010
filing date -
Nov 3, 2009
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Abandoned
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Abstract
The present invention provides a new approach, quantitative Nuclease Protection Sequencing (qNPS™), for addressing several challenges that face sequencing and which provides improvements for research and diagnostic applications. The method uses a lysis-only nuclease protection assay to generate nucleic acid, e.g., DNA probes for sequencing, which can be coupled to gene-specific tags to permit the identification of the gene without necessitating the sequencing of the nuclease protection probe itself and/or can be coupled to experiment-specific tags whereby samples from different patients can be combined into a single run. The disclosed qNPS makes sequencing fixed or insoluble samples possible and affordable as a research and discovery tool and as a diagnostic test.
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