METHOD AND INTRODUCTION OF GENE INTO YEAST CELL, AND VECTOR FOR THE METHOD
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United States of America Patent
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Issued Date -
Apr 7, 2011
app pub date -
May 11, 2009
filing date -
May 14, 2008
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Abandoned
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Abstract
An object of the present invention is a method for introducing a foreign gene into a yeast cell that does not have an auxotrophic marker. The present invention provides a method for providing a target auxotrophy to a yeast cell and introducing a gene to be expressed into the yeast cell. The method includes the step of transforming a yeast cell with a fragment containing an expression cassette for the gene to be expressed, a cassette for a yeast selectable marker, and two homologous recombination fragments each homologous to a region on either side of a target auxotrophy controlling gene. According to the method, a target auxotrophy controlling gene is deleted from a yeast cell and a gene to be expressed is introduced into the yeast cell, and further the yeast selectable marker is eliminated from the transformed yeast cell.
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