Polymerases for nucleotide analogue incorporation
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United States of America Patent
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app pub date -
Jun 8, 2010
filing date -
Dec 22, 2005
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Abstract
Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.
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