Method for Commercial Isolation of Egg Yolk IgY, Its Product, and Uses of the Product
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United States of America Patent
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Issued Date -
Jan 20, 2011
app pub date -
Jul 16, 2009
filing date -
Jul 16, 2009
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Abandoned
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Abstract
A method for extraction and purification of IgY from immune avian egg yolk comprising diluting a mixture of liquid egg yolk and liquid egg white in treated water; stirring the diluted mixture to obtain an egg mixture; separating the egg mixture into a first water soluble fraction, an unseparated mixture fraction, and a first separated lipophilic fraction;separating the first water soluble fraction; the unseparated mixture fraction, and the first separated lipophilic fraction; centrifuging the unseparated mixture fraction until it separates into a second water soluble fraction and a second separated lipophilic fraction; adding the second water soluble fraction to the first water soluble fraction to form a combined water soluble fraction; passing the combined water soluble fraction through an ion exchange adsorption column containing IgY-adsorbent beads to adsorb IgY; eluting the adsorbed IgY of from the ion exchange adsorption column with an eluting solution to obtain an eluant; filtering the eluant of through a first membrane filtration system to obtain a 4% to 25% IgY concentrate; and drying the IgY concentrate to obtain a 40% to 98% IgY isolate, as well as a food-safe IgY isolate obtained and use of a food-safe IgY isolate in an edible product.
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