NUCLEIC ACID SIGNAL ENHANCEMENT USING NANOPARTICLE-BASED HYBRIDIZATION
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United States of America Patent
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Issued Date -
Jan 20, 2011
app pub date -
Jan 14, 2010
filing date -
Jan 14, 2009
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Abandoned
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Abstract
A method of enhancing signal detection through use of nanoparticle-conjugated nucleic acid probes is provided. Following chromosomal FISH hybridization of a target sequence with a genomic probe linked to a flag sequence, the flag sequence is hybridized to an anti-flag sequence conjugated to a nanoparticle. The enhanced fluorescent probe is then visualized using microscopy.
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