NUCLEIC ACID PURIFICATION METHOD
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United States of America Patent
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Issued Date -
Dec 30, 2010
app pub date -
Jul 23, 2008
filing date -
Jul 27, 2007
priority date (Note) -
Abandoned
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Abstract
The invention provides an modified method for the separation of nucleic acids from cells, comprising: generating an aqueous solution containing the nucleic acid by lysing the cells with a lysis solution including SDS and salt; and separating the nucleic acids of interest from other cellular components. The improvement includes adding a non-ionic detergent in the lysis solution such that SDS is not precipitated and no heating of the solution is required prior to cellular lysis. The preferred non-ionic detergents are the polysorbate family of compound, including TWEEN® 20. Also disclosed are composition and kit for performing the modified method.
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