PROCESS FOR THE STABLE GENE INTERRUPTION IN CLOSTRIDIA
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United States of America Patent
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Issued Date -
Dec 30, 2010
app pub date -
Jun 25, 2010
filing date -
Jun 26, 2009
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Abandoned
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Abstract
The present invention is related to a new method for interrupting multiple DNA sequences in Clostridia, even in genes recognized to be essential for the optimal growth of Clostridii by using a counter-selectable marker that would pinpoint the cells that have lost the plasmid and acquired a modified function that permits survival without the interrupted gene. This method is easy to perform and applicable at an industrial level. This method is useful to modify several genetic loci in Clostridia in a routine manner. This method is based on a replicative vector carrying at least two marker genes.
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