FEEDER CELL-FREE CULTURE MEDIUM AND SYSTEM
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United States of America Patent
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Issued Date -
Dec 16, 2010
app pub date -
Sep 3, 2008
filing date -
Sep 4, 2007
priority date (Note) -
Abandoned
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Abstract
A cell culture medium and system are provided which eliminates or at least reduces the need for feeder cells. The cell culture medium comprises one or more factors that are normally secreted and/or produced by a feeder cell and a synthetic chimeric protein comprising IGF-I and a portion of vitronectin. The cell culture medium is particularly suitable for propagating human embryonic stem cells and keratinocytes. This invention also relates to compositions and methods which utilize the cells cultured in the cell culture medium of the invention.
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