SYSTEM FOR TRANSPOSING HYPERACTIVE RECOMBINANT DERIVATIVES OF MOS-1 TRANSPOSON
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United States of America Patent
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Issued Date -
Nov 18, 2010
app pub date -
May 15, 2007
filing date -
May 15, 2006
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Abandoned
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Abstract
The invention concerns a system for transposing a hyperactive recombinant derivative of Mos-1 transposon, comprising at least the two following partners: a) a Mos-1 pseudo-transposon in which an exogenous nucleotide sequence of interest replaces the nucleotide sequence encoding the original Mos-1 transposase; and b) a Mos-1 tranposase provided in trans in said pseudo-transposon, at least one of said partners being appropriately genetically modified to improve the transposition frequency of said exogenous nucleotide sequence of interest. Additionally to such systems, the invention concerns hyperactive Mos-1 transposons, hyperactive Mos-1 transposases, kits. The invention further concerns the use of one or more of abovementioned means for carrying out sequence transpositions, and more particularly efficient gene transfers.
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