HIGH GRADIENT MAGNETIC SEPARATION OF BIOLOGICAL MATERIAL
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United States of America Patent
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Issued Date -
Oct 28, 2010
app pub date -
Aug 26, 2008
filing date -
Sep 11, 2007
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Abandoned
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Abstract
The invention relates to a high gradient magnet separation device for the separation or purification of magnetic or magnetically labeled biological material, comprising a magnet, a separating column, and a ferromagnetic matrix that can be arranged in an interior chamber of the separating column, and a storage container containing a buffer solution for the equilibration of the separating column and/or for the suspension of the biological material, wherein during operation a magnetic field created by the magnet creates a high gradient magnetic filed in the matrix and buffer solution can flow from the storage container through the separating column. For this purpose, the buffer solution comprises a base solution and macromolecules that can saturate nonspecific binding sites of the matrix.
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