METHOD FOR SPECIFICALLY AND SENSITIVELY AMPLIFYING A TARGET SEQUENCE
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United States of America Patent
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Oct 14, 2010
app pub date -
Dec 28, 2006
filing date -
Dec 28, 2005
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Abstract
The present invention aims to develop a method for amplifying only a target gene while reducing the noise from a non-target gene having a similar sequence, as well as to develop a new method of quantitation based on acquisition of the signal from the amplified sequence with high precision (in high sensitivity and specificity).As a result of their intensive studies, the present inventors revealed that the stated object could be attained by incorporating a third primer in the PCR reaction mixture and this has led to the accomplishment of the present invention.
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