BIOASSAY FOR GENE SILENCING CONSTRUCTS
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United States of America Patent
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Issued Date -
Oct 7, 2010
app pub date -
Apr 4, 2010
filing date -
Apr 3, 2009
priority date (Note) -
Abandoned
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Abstract
The invention provides constructs and methods of screening for constructs useful in conferring resistance in plants to pests by gene silencing. The invention also provides pest-resistant plants transformed with the present constructs. One screening method of the invention comprises the steps of: selecting at least one pest target nucleotide sequence, producing a plurality of dsRNA test agents that target the pest target nucleotide sequence, testing and scoring the plurality of dsRNA test agents for toxicity to the pest, and producing a silencing construct based on a superior-scoring test agent.
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