Method and Kit for Measurement of Acrolein Adduct in Sample Utilizing Agglutination Reaction of Immunological Microparticle
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United States of America Patent
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Issued Date -
Sep 23, 2010
app pub date -
Oct 22, 2008
filing date -
Oct 22, 2007
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Abandoned
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Abstract
A method for measuring an acrolein adduct present in a sample comprising the steps of: (a) mixing a sample containing the acrolein adduct with a solution comprising an immunoglobulin that specifically recognizes the acrolein adduct; (b) adding to the mixture obtained in the step (a), a solution comprising microparticles to which a substance that specifically binds to the immunoglobulin that specifically recognizes the acrolein adduct and a blocking agent have been bound, and mixing them; and (c) measuring an extent of an agglutination reaction of the microparticles in the mixture obtained in the step (b), wherein the extent of the agglutination reaction being decreased relative to an amount of the acrolein adduct in the sample. The method enables a measurement of an acrolein adduct without requiring a complex operation.
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