METHOD FOR ISOLATING HOMOGENEOUS POPULATIONS OF TRANSDUCED PROGENITORS STABLY EXPRESSING SPECIFIC LEVELS OF A TRANSGENE
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United States of America Patent
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Issued Date -
Sep 23, 2010
app pub date -
May 16, 2007
filing date -
May 17, 2006
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Abandoned
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Abstract
, via an IRES sequence accommodating translation of the target and the marker genes from the same mRNA, then correlating an expression level of the target gene with that of the cell-surface marker gene, selecting a reference clone of cultured precursor cells expressing the target gene at the desired threshold, and isolating precursor cells having the same or similar level of expression of the target gene than the reference clone.
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