rbLIF PROTEIN FOR USE IN EMBRYONIC STEM CELL CULTURES
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United States of America Patent
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Issued Date -
Sep 9, 2010
app pub date -
Dec 14, 2009
filing date -
Dec 15, 2008
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Abandoned
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Abstract
Provided herein methods of maintaining an embryonic stem cell culture in an undifferentiated state, wherein the method includes culturing an embryonic stem cell on a rabbit feeder cell layer, wherein the rabbit feeder layer expresses a leukemia inhibitory factor (LIF) protein. Also provided are methods of maintaining an embryonic stem cell culture in an undifferentiated state, wherein the method includes culturing an embryonic stem cell in a culture medium comprising rabbit leukemia inhibitory factor (rbLIF) protein. Also provided are polynucleotide and amino acid sequences of a rabbit leukemia inhibitory factor protein (rbLIF).
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