MEDIA AND PROCESSES FOR THE EX VIVO PRODUCTION OF MEGAKARYOCYTES FROM HUMAN CD34+ CELLS
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United States of America Patent
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Apr 5, 2010
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Aug 6, 2003
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Abstract
cells, either being freshly isolated from a newborn's cord blood or having been subcultured in an expansion medium after isolation from a newborn's cord blood, are subjected to cultivation in a cultivating medium consisting essentially of a basal medium, a serum substitute and a cytokine cocktail, wherein the serum substitute consists of human serum albumin, insulin, and transferrin; and the cytokine cocktail consists of thrombopoietin (TPO), stem cell factor (SCF), Flt-3 ligand (FL), interleukin-3 (IL-3), IL-6, IL-9, and granulocyte-macrophage colony-stimulating factor (GM-CSF).
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