Method for Incorporating Proteins Into Lentivirus Vectors
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United States of America Patent
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Issued Date -
Sep 2, 2010
app pub date -
Feb 11, 2008
filing date -
Feb 12, 2007
priority date (Note) -
Abandoned
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Abstract
The present invention is a method for incorporating an integrase-fusion protein into a third-generation lentivirus vector, comprising: (i) transfecting a vector packaging plasmid into a producer cell, wherein the vector packaging plasmid contains a lentivirus transfer construct and a gene encoding the integrase-fusion protein, said gene being fused to the pol-polyprotein gene; (ii) transcription and translation of the genes; and (iii) release of the integrase-fusion protein from the pol-polyprotein.
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