ISOTHERMAL AMPLIFICATION METHOD AND DNA POLYMERASE USED IN THE SAME
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United States of America Patent
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Issued Date -
Sep 2, 2010
app pub date -
Oct 24, 2008
filing date -
Oct 25, 2007
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Abandoned
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Abstract
A DNA polymerase suitable for specific isothermal amplification methods and an isothermal amplification method using the DNA polymerase are provided. In the presence of a DNA polymerase including a protein described in the following item (a) or (b), an amplification reaction of a target nucleic acid sequence in a nucleic acid sample is carried out isothermally using a first primer shown in the following (X). By using the DNA polymerase, it becomes possible to carry out the amplification reaction using the primer within a shorter time than ever before. (a) a protein having an amino acid sequence represented by SEQ ID NO. 23 (b) a protein having an amino acid sequence represented by SEQ ID NO. 25 (X) a primer that contains, in a 3' end portion, a sequence (Ac') that hybridizes to a sequence (A) of a 3' end portion of the target nucleic acid sequence and also contains, on a 5' side of the sequence (Ac'), a sequence (B') that hybridizes to a complementary sequence (Bc) to a sequence (B) present on a 5' side with respect to the sequence (A) in the target nucleic acid sequence
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