FROZEN CELL IMMOBILIZED PRODUCT, PRIMARY HEPATOCYTE CULTURE TOOL, AND METHOD FOR PRODUCING PRIMARY HEPATOCYTE CULTURE TOOL
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United States of America Patent
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Issued Date -
Aug 26, 2010
app pub date -
Sep 11, 2008
filing date -
Oct 19, 2007
priority date (Note) -
Abandoned
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Abstract
The present invention provides a frozen cell immobilized product which is obtained by applying a technique for freezing cultured cells to a technique for forming primary hepatocyte spheroids through co-culturing, and which can improve performance in an examination or a test using the technique for forming primary hepatocyte spheroids; a primary hepatocyte culture tool; and a method for producing the primary hepatocyte culture tool. According to the invention, a cell-adhesion region of a culture substrate is defined in a pattern; animal-derived adherent cells are cultured on the cell-adhesion region; and the cultured cells are frozen together with a freezing culture medium.
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