METHOD OF DETERMINING CARBONIC ANHYDRASE I ACTIVITY
Number of patents in Portfolio can not be more than 2000
United States of America Patent
Stats
-
N/A
Issued Date -
N/A
app pub date -
Apr 28, 2010
filing date -
Apr 5, 2004
priority date (Note) -
Abandoned
status (Latency Note)
Importance
Abstract
A method for determining hydrolase activity of carbonic anhydrase I (CAI) in a sample which employs, combination of a substrate and an inhibitor. The substrate is a substrate having higher reactivity with CAI than with CAII selected from 2-hydroxy-5-nitro-α-toluenesulfonic acid sultone, a o-nitrophenyl ester, a p-nitropheylthio ester, and a β-naphthyl ester or a substrate having reactivity with both CAI and CAII selected from the group consisting of a p-nitrophenyl ester and a α-naphthyl ester. The substrate having higher reactivity with CAI than with CAII is a substrate that reacts with CAI in an amount, per amount of enzyme protein, twice or more the amount of substrate reacting with CAII, under identical substrate concentrations and reaction times and that specifically binds to CAI and serves as a substrate for hydrolase activity. The inhibitor is an inhibitor inhibiting a hydrolase other than CA, a CA inhibitor inhibiting both CAI and CAII, or a CA inhibitor inhibiting CAI more potently than CAII.
First Claim
Family
International Classification(s)
Cited Art Landscape
Patent Citation Ranking
Recipient Email Address
Recipient Email Address
Comment
Recipient Email Address
Add to Portfolio(s)
To add this patent to one, or more, of your portfolios, simply click the add button.
This Patent is in these Portfolios:
Add to additional portfolios:
Last Refreshed On:
Changes done successfully