VECTORS AND METHODS FOR ENZYME PRODUCTION
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United States of America Patent
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Issued Date -
Jun 17, 2010
app pub date -
Mar 6, 2008
filing date -
Mar 6, 2007
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Abandoned
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Abstract
The invention provides a method for producing a replicase EF-Ts, EF-Tu and β polypeptide subunits, the method comprising expressing in a suitable host cell the EF-Ts and EF-Tu subunits, followed by expressing the β subunit, wherein the EF-Ts and EF-Tu subunits are operably linked to a first promoter and the β subunit is operably linked to a second promoter, and wherein the first and second promoters are differentially induced.
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