Method for Identifying the Genotype in Position 171 of the Ovine Prion Protein as well as Kits for Implementing said Method
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United States of America Patent
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Issued Date -
Jun 17, 2010
app pub date -
Nov 24, 2006
filing date -
Nov 25, 2005
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Abandoned
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Abstract
The invention relates a method to identify genotype at position 171 of the ovine PrP, and a method to select an ovine for reproduction.It also relates kits to implement these methods.The identification method of the invention comprises the steps of treating a sample of ovine biological fluid to be tested containing the PrP with a denaturing and reducing solution, immobilizing the denatured and reduced PrP, optionally via a ligand, on a solid phase, contacting the denatured, reduced and immobilized PrP with at least one detection antibody, and of detecting the possible presence of said at least one detection body, one of the ligand or of said at least one detection antibody specifically binding with the PrP having a particular allelic form at position 171.The invention finds particular application in the medical field, more particularly veterinary and sanitary fields.
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