IDENTIFICATION OF PHOSPHORYLATION SITES IN POLYPEPTIDES BY EMPLOYMENT OF URANYL PHOTOCLEAVAGE
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United States of America Patent
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Issued Date -
Jun 10, 2010
app pub date -
Dec 7, 2007
filing date -
Dec 7, 2006
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Abandoned
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Abstract
The present invention relates to a method of cleaving a polypeptide at one or more phosphorylated residues. Said cleavage is induced by irradiation and is dependent on the presence of uranyl. The method is useful for analysis of phosphoproteoms and also for protein purification. The method also relates to a method of protein purification, wherein the phosphorylated protein is immobilized on a column said immobilization being dependent on uranyl.
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