Disclosed is a registration method of tissue position and an apparatus using the method. The apparatus according to the present invention comprises an image storage unit storing an examined image associated with a target bone of surgery, a first position measuring unit measuring position values of multiple points on a surface of an exposed bone, a second position measuring unit acquiring an image of the exposed bone, and a registration control unit for performing a first registration between the positions obtained from the examined image associated with the target bone of surgery and the positions measured by the first position measuring unit, and for performing a second registration between the positions of the image acquired as a result of the first registration and the positions of the image acquired by the second position measuring unit.

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