APPARATUS AND METHOD FOR MEASURING THE SIGNAL FROM A FLUORESCING NANODROP CONTAINED BY SURFACE TENSION
Number of patents in Portfolio can not be more than 2000
United States of America Patent
Stats
-
N/A
Issued Date -
May 27, 2010
app pub date -
Feb 9, 2006
filing date -
Feb 11, 2005
priority date (Note) -
Abandoned
status (Latency Note)
Importance
Abstract
Apparatus and method for measuring the fluorescence of nanodrop liquid samples is described in which the sample is held by surfa tension between two anvil surfaces (20, 24). Each anvil surface (20, 24) has an embedded optical fiber (18) with its end finished fl with the surface in the containment area wetted by the sample with the fiber (18) in line. Sample excitation is provided from the sid of the sample remote from the containment area. By selection of the fiber transmission numeric aperture the impact of exciting and ambient light on the measurement is minimized. A method of virtual filtering is taught in which any ambient or exciting light that does impinge on the measuring sensor is corrected by subtracting a scaled representation of the source from the measurement. The method and apparatus is capable of detecting (1) femptomole of sodium fluorescein in (1) microliter of TE buffer.
First Claim
Family
International Classification(s)
Cited Art Landscape
Patent Citation Ranking
Recipient Email Address
Recipient Email Address
Comment
Recipient Email Address
Add to Portfolio(s)
To add this patent to one, or more, of your portfolios, simply click the add button.
This Patent is in these Portfolios:
Add to additional portfolios:
Last Refreshed On:
Changes done successfully