AMPLIFICATION OF SINGLE VIRAL GENOMES
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United States of America Patent
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Issued Date -
Feb 25, 2010
app pub date -
Aug 18, 2009
filing date -
Aug 18, 2008
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Abandoned
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Abstract
The present invention relates, e.g., to a method for amplifying the genome of a single virus particle from a mixture of virus particles, comprising (a) subjecting the mixture of virus particles to flow cytometry and identifying a sorted sample that putatively contains a single virus particle, (b) imbedding the sorted sample comprising the putative single viral particle in a solid matrix (e.g., low melting agarose); (c) visualizing the embedded virus particle (e.g., by EFM and/or confocal microscopy) to confirm that a single particle is embedded; and (d) exposing the nucleic acid from the visualized, embedded single, discrete viral particle (e.g., by alkali treatment) and amplifying the genomic viral nucleic acid in situ (e.g., by MDA).
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