WAY TO OBTAIN HIGH EXPRESSION CLONES OF MAMMALIAN CELLS USING A METHYLCELLULOSE WITH FLUORESCENT PROTEIN A OR G AND FLUORESCENT SCREENING METHOD
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United States of America Patent
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Issued Date -
Feb 4, 2010
app pub date -
Mar 28, 2008
filing date -
Mar 30, 2007
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Abandoned
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Abstract
The invention provides a genetic screening method for identifying a transfected cell expressing the polypeptide of interest. The methods allows for high throughput screening of recombinant cells for elevated levels of expression of the polypeptide of interest using methylcellulose comprising fluorescent protein A or G to improve detection and cloning. The invention also provides capture media, formulations and methods of making and using thereof.
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