COMPOSITIONS, METHODS, AND KITS FOR ANALYZING DNA METHYLATION
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United States of America Patent
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Issued Date -
Feb 4, 2010
app pub date -
Jul 6, 2009
filing date -
Feb 18, 2005
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Abstract
Compositions, methods, and kits for reducing strand amplification bias using bisulfite treated gDNA are provided. Methods for detecting and for quantitating the amplified bisulfite treated gDNA and inferring the presence, absence, and/or degree of methylation of target cytosine(s) in the gDNA are also provided. Such methods typically employ tailed first primer pairs, which can, but need not comprise nucleotide analogs, and optionally second primer pairs.
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