METHODS AND MEANS FOR NUCLEIC ACID SEQUENCING
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United States of America Patent
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Issued Date -
Feb 4, 2010
app pub date -
Mar 14, 2007
filing date -
Mar 14, 2006
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Abstract
The present invention provides a nucleic acid sequencing method. The method comprises enriching a nucleic acid sample for target nucleic acids, where the nucleic acid sample is enriched through at least a first round of hybridization selection and amplification, and a second round of hybridization selection and amplification. The enriched nucleic acids are in a form convenient for sequencing with the Cantaloupe sequencing technology, which employs shotgun sequencing by hybridization (SBH) of immobilized rolling circle amplicons.
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