SYSTEM AND METHOD TO PREVENT CROSS-CONTAMINATION IN ASSAYS PERFORMED IN A MICROFLUIDIC CHANNEL
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United States of America Patent
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Issued Date -
Dec 31, 2009
app pub date -
Jun 30, 2008
filing date -
Jun 30, 2008
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Abstract
The present application discloses systems and methods for preventing contamination in assays performed in microfluidic channels. In one embodiment, a buffer of non-reactive fluid is provided between an input port and a microchannel in which assays are performed during such times that flow from the input port is stopped. In general, an amount of non-reactive fluid is drawn into a channel connecting the stopped input port to the microchannel. Thus, any seepage, or diffusion, from the channel connecting the stopped input port to the microchannel will be of the non-reactive fluid, not the reagent, or other potentially-contaminating fluid, introduced through the input port. In one embodiment, microvalves and a negative pressure differential source control flow of reagents into the microchannel and the flow of non-reactive fluid into the inlet conduits.
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