METHOD FOR TESTING DRUG SENSITIVITY IN SOLID TUMORS BY QUANTIFYING MRNA EXPRESSION IN THINLY-SLICED TUMOR TISSUE
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United States of America Patent
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Issued Date -
Dec 3, 2009
app pub date -
May 8, 2007
filing date -
May 8, 2006
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Abstract
A method is disclosed for assaying the sensitivity of neoplastic tissue to therapeutic agents, and in particular, for the quantification of pro-apoptotic marker mRNA expression in cells obtained from thinly-sliced living tumor tissue in such methods. The method may comprise ascertaining a particular apoptosis marker mRNA for an individual tumor or tumor type as well as exposure of thin-sliced live cancer tissues from the individual tumor to candidate chemotherapeutic drug regimes in vitro, followed by an assessment of the level of the marker mRNA in the tissue.
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